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Effect of shear stress on the permeability coefficient of sucrose and mannitol in a microfluidic chip. (A) Note the effect of shear stress on the permeability of both paracellular markers. (B) Representative immunofluorescence images of iBMEC monolayers fixed and stained after 48 h at 0, 0.15, 1.5, and 3 dyne/cm2. ZO-1 (red) and F-actin phalloidin (green). n = 3 biological replicates. * p < 0.05 and ** p < 0.001 assessed by one-way ANOVA, followed by Tukey’s multiple comparisons test.
