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. 2021 Aug 24;10(9):2181. doi: 10.3390/cells10092181

Figure 1.

Figure 1

E2 enhances angiogenesis effects in Matrigel® plugs containing MSCs. (A) Exhibits a macroscopic view of Matrigel® plugs from each group—sham-operated or OVX—in presence or absence of E2 (100 nM): C57Bl mice injected with Matrigel® only, Matrigel® + cells (Matrigel® + MSC), or Matrigel® + hormone-treated cells (Matrigel® + MSC + E2). (B) Displays measures of hemoglobin content using Drabkin’s reagent kit of Matrigel® implants from both groups. The levels of this angiogenesis marker were amplified in MSCs stimulated with the sex hormone (MSC + E2 ± OVX) in comparison with control (MSCs only). Each value corresponds to mean ± SEM (n = 3); * p < 0.05, ** p < 0.01 compared with control. (C) Descriptive photomicrographs of Matrigel®-derived blood capillaries/vessels embedded in paraffin and stained with hematoxylin & eosin, and DAPI; Orange arrows indicate blood vessel structures; scale bar—100 μm. (D) Quantification of the new blood vessels was presented as mean ± SD (n = 2), ** p < 0.01, and * p < 0.001 compared with control. Note: E2, 17-beta estradiol; MSCs, Wharton’s jelly-derived mesenchymal stem cells; OVX, ovariectomy surgery.