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. 2021 Sep 18;11(9):637. doi: 10.3390/metabo11090637

Figure 2.

Figure 2

Breast cancer cell lines display a heterogeneous response to perturbations of the autophagic flux. (A) Variations in the autophagy sensor were monitored in three breast cancer cell lines upon different autophagy regulators treatment in the supplemented medium. RAP, TOR and SPA effect over the autophagy flux was measured after 24 h of treatment of the respective drug, then the medium was replaced with new supplemented culture medium containing the respective same drug concentration. WRT and HCQ activities over the autophagy flux are immediate, for that reason their effect over this pathway was quantified immediately upon treatment. (B) Variations in the autophagy sensor were monitored in three breast cancer cell lines upon different autophagy regulators treatment in starvation medium. SPA treatment began 24 h before the kinetic assay, then the supplemented medium containing this treatment was replaced with a starvation medium containing the same drug dose. No pretreatment was performed with WRT and HCQ. HCQ: hydroxychloroquine, RAP: rapamycin, SPA: Spautin-1, TOR: Torin-2, WRT: wortmannin.