Table 2.

Sequences of the cytokine-specific primers and probes.

Cytokine	Specific Primer Sets (5′→3′)	Probe	Length (bp)	Reference
IFN-γ	F-CGATCCTAAAGGACTATTTTAATGCAA R-TTTTGTCACTCTCCTCTTTCCAAT	ACCTCAGATGTACCTAATGGTGGACCTCTT	102	[36]
IL-4	F-GTCTGCTTACTGGCATGTACCA R-GCTCCATGCACGAGTTCTTTCT	CCACGGACACAAGTGCGACATCACCTTAC	117	[37]
IL-10	F-CGGCGCTGTCATCAATTTCTG R-CCCCTCTCTTGGAGCTTGCTA	AGGCACTCTTCACCTCCTCCACGGC	89	[36]
GAPDH	F-ACATGGCCTCCAAGGAGTAAGA R-GATCGAGTTGGGGCTGTGACT	CCACCAACCCCAGCAAGAGCACGC	105	[37]

FAM 5′ was used for IFN-γ, IL-4, IL-10, and GAPDH; BHQ (Black Hole Quencher)-1 was used to target the 3′ end of IFN-γ, IL-4, IL-10, and GAPDH. F: forward primer; R: reverse primer.