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The CAR peptide co-localized to damaged (indicated by EBD staining) muscle cells in the mdxD2 mice. The quadriceps of these mice were directly injected with CAR-FITC peptide and harvested 2 h later. The EBD positive fiber demonstrated co-localization with FITC coupled CAR peptide (arrow). Slides were fluorescently visualized with: (A) EBD for membrane permeability, (B) directly for the FITC peptide, (C) DAPI for the nuclei, and (D) merged images. Original magnification was 40×.
