Figure 4.

CAR-DCN localized to damaged muscle cells in the ɣ-sarcoglycan null quadriceps muscles, a second model of muscular dystrophy. (A) Similar to the mdx model injected CAR-DCN co-localized to a subset of damaged muscle tissues. The sgcgD2 mice received three weeks of IP injections every other day. Slides were visualized with EBD for membrane permeability (red, first column) and anti-histidine antibodies for the recombinant proteins (green, middle column), and last column shows merged images. In many EBD positive fibers, CAR has targeted to the sarcolemma (asterisks, top and middle rows). In other EBD positive fibers, no His-tag staining for the recombinant protein was detected (arrows). (B) The DCN protein containing mutant CAR (mCAR-DCN) never co-localized to damaged cells. Original magnification was 10×.