Figure 5.

Three-weeks of CAR-DCN treatment decreased p-SMAD2/3 staining in the SgcgD2 mouse model. p-SMAD2/3 specific antibodies followed by species specific secondary antibody were used to detect nuclear and therefore activated p-SMAD2/3. Although these animals were not injected with EBD (to reserve the robust red channel for low-abundance p-SMAD staining), the damaged fibers can be identified because of the variable fiber size (*) and central nuclei (arrowheads). All panels are from SgcgD2 quadriceps injected with either CAR-DCN or mCAR-DCN. Original magnification 40×.