Three weeks of CAR-DCN treatment resulted in decreases in α smooth muscle actin staining in quadriceps muscle. SgcgD2 quadriceps were stained with dystrophin and α smooth muscle actin. The blood vessels from CAR-DCN treated mice had staining for α smooth muscle actin (white arrows, top four panels), but did not have extravascular staining (i.e., myofibroblasts). The PBS control quadriceps had both vessel staining (white arrows) and extracellular staining (red arrowheads, bottom four panels) indicating myofibroblast transformation. In addition, the dystrophin staining appeared more uniform in the CAR-DCN treated mice. Original magnification 40×.