Figure 1.

Indole is produced during growth in sub-MIC tobramycin and induces raiA

Measure of extracellular indole concentrations of bacterial cultures grown overnight in rich medium MOPS (Teknova EZ rich defined medium) with and without tobramycin at 0.10 μg/mL (TOB 0.10) or 0.15 μg/mL (TOB 0.15) using the Kovacs reagent (Saint-Ruf et al., 2014). ΔtnaA strain was used as a negative control without tobramycin. Experiments were performed in triplicates, and statistical analysis was performed (∗∗∗: p < 0.001). Error bars represent standard deviation.