REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat polyclonal anti-mouse IgG with HRP	Merck Millipore	Cat# AP503P RRID: AB_805355
Goat polyclonal anti-mouse IgG1 with HRP	SouthernBiotech	Cat# 1070-05; RRID: AB_2650509
Goat polyclonal anti-mouse IgG2b with HRP	SouthernBiotech	Cat# 1090-05; RRID: AB_2794521
Goat polyclonal anti-mouse IgG2c with HRP	SouthernBiotech	Cat# 1079-05; RRID: AB_2794466
Anti-mouse CD16/CD32 (clone: 93)	BioLegend	Cat# 101302; RRID: AB_312801
BV421 anti-mouse CD45 (clone: 30-F11)	BioLegend	Cat# 103134; RRID: AB_2562559
FITC anti-mouse Ly6G (clone: 1A8)	BioLegend	Cat# 127605; RRID: AB_1236488
BV785 anti-mouse CD11b (clone: M1/70)	BioLegend	Cat# 101243; RRID: AB_2561373
APC anti-mouse CD3 (clone: 145-2C11)	BioLegend	Cat# 100312; RRID: AB_312677
PE/Cy7 anti-mouse CD4 (clone: RM4-5)	BioLegend	Cat# 100528; RRID: AB_312729
PerCP anti-mouse CD8a (clone: 53-6.7)	BioLegend	Cat# 100731; RRID: AB_893427
APC/Cy7 anti-mouse CD11c (clone: N418)	BioLegend	Cat# 117324; RRID: AB_830649
APC anti-mouse Siglec-F (clone: REA798)	Miltenyi Biotec	Cat# 130-112-333 RRID: AB_2653441
PE anti-mouse FcγRI (CD64; clone: X54-5/7.1)	BioLegend	Cat# 139303; RRID: AB_10613467
PE mouse IgG1 κ isotype control (clone: MOPC-21)	BioLegend	Cat# 400111
PE anti-mouse FcγRIIb (CD32b; clone: AT130-2)	Invitrogen	Cat# 12-0321-82; RRID: AB_2572557
PE mouse IgG2a κ isotype control (clone: eBM2a)	Invitrogen	Cat# 12-4724-82; RRID: AB_470064
PE anti-mouse FcγRIII (CD16; clone: 275003)	R&D Systems	Cat# FAB19601P-100 RRID: AB_2246942
PE rat IgG2a isotype control (clone: 54447)	R&D Systems	Cat# IC006P RRID: AB_357256
PE anti-mouse FcγRIV (CD16.2; clone: 9E9)	BioLegend	Cat# 149503; RRID: AB_2565810
PE american hamster IgG isotype control (clone: HTK888)	BioLegend	Cat# 400907 RRID: AB_326593
Rat IgG2b κ isotype control (clone: RTK4530)	BioLegend	Cat# 400672
Rat IgG2a isotype control (clone: 2A3)	BioXcell	Cat# BE0089; RRID: AB_1107769
Anti-IFN-γ antibody (clone: XMG1.2)	BioXcell	Cat# BE0055; RRID: AB_1107694
Rat IgG1 isotype control (clone: HRPN)	BioXcell	Cat# BE0088; RRID: AB_1107775
Antagonistic monoclonal antibody against FcγRIIb (clone: AT128)	Dr. Mark S. Cragg, University of Southampton	N/A
Mouse IgG1 isotype control (clone: MOPC-21)	BioXcell	Cat# BE0083; RRID: AB_1107784
Agonistic monoclonal antibody against FcγRIIb (clone: AT130-2)	Dr. Mark S. Cragg, University of Southampton	N/A
Mouse IgG2a isotype control (clone: C1.18.4)	BioXcell	Cat# BE0085; RRID: AB_1107771
BV711 anti-mouse IFN-γ (clone: XMG1.2)	BioLegend	Cat# 505836; RRID: AB_2650928
PE-Cy7 anti-mouse IL-13 (clone: eBio13A)	Thermo Fisher Scientific	Cat# 25-7133-80; RRID: AB_2573530
FITC anti-mouse CD4 (clone: GK1.5)	BioLegend	Cat# 100406; RRID: AB_312691
BV605 anti-mouse CD8 (clone: 53-6.7)	BioLegend	Cat# 100744; RRID: AB_2562609
AF647 anti-mouse CD45 (clone: 30-F11)	BioLegend	Cat# 103124; RRID: AB_493533
PE anti-mouse CD3 (clone: 145-2C11)	BioLegend	Cat# 100308; RRID: AB_312673
Bacterial and virus strains
Ether-treated hemagglutinin-antigen-enriched virion-free split vaccine (SV) from H1N1 influenza virus (strain: A/California/7/2009 (Cal7))	Dr. YasuyukiGomi, The Research Foundation for Microbial Diseases of Osaka University	N/A
Formalin-inactivated whole-virion vaccine (WV) from H1N1 influenza virus (strain: Cal7)	Dr. YasuyukiGomi, The Research Foundation for Microbial Diseases of Osaka University	N/A
H1N1 influenza virus (strain: A/Puerto Rico/8/34 (PR8))	Dr. YasuyukiGomi, The Research Foundation for Microbial Diseases of Osaka University	N/A
H1N1 influenza virus (strain: Cal7)	Dr. Hideki Asanuma, National Institute of Infectious Diseases	N/A
Chemicals, peptides, and recombinant proteins
Recombinant HA proteins derived from Cal7	Kawai et al., 2020	N/A
Recombinant HA proteins derived from PR8	Kawai et al., 2020	N/A
Alhydrogel Adjuvant 2%	InvivoGen	Cat# vac-alu-250
Block Ace	DS Pharma Biomedical	Cat# UKB80
Tetramethyl Benzidine	Nacalai Tesque	Cat# 05299-54
Receptor-destroying Enzyme (RDE) (II)	Denka Seiken	Cat# 340016
Amido Black	Nacalai Tesque	Cat# 01927-92
Guinea Pig Red Blood Cells	Japan Bio Serum	Cat# 035-00012
7-amino-actinomycin D (7-AAD)	BioLegend	Cat# 420404
Recombinant Mouse IFN-γ	BioLegend	Cat# 575306
Clophosome®-A-Clodronate Liposomes (anionic)	FormuMax Scientific Inc.	Cat# F70101C-A
Plain Control Liposomes (Anionic)	FormuMax Scientific Inc.	Cat# F70101-A
Protein Transport Inhibitor Cocktail	Thermo Fisher Scientific	Cat# 00-4980-93
Brefeldin A	Sigma-Aldrich	Cat# B6542
Collagenase Type IV	Gibco	Cat# 17104-019
Deoxyribonuclease 1	Wako	Cat# 047-26771
HiTrap Protein G HP	GE Healthcare	Cat# 17040403
HiTrap Protein A HP	GE Healthcare	Cat# 29048576
Fixable Viability Dye eFluor 780	Thermo Fisher Scientific	Cat# 65-0865-14
Critical commercial assays
CD4+ T cell Isolation Kit	Miltenyi Biotec	Cat# 130-104-454
Expi293™ GnTI- Expression System Kit	Thermo Fisher Scientific	Cat# A39250
IFN-γ ELISA Kit	BioLegend	Cat# 430801
IL-13 ELISA Kit	R&D Systems	Cat# DY413-05
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat# 23225
Fixation/Permeabilization Solution Kit with BD GolgiPlug	BD Biosciences	Cat# 555028
Experimental models: Cell lines
Madin-Darby Canine Kidney (MDCK) cells	The Research Foundation for Microbial Diseases of Osaka University	N/A
GK1.5 (Anti-CD4 antibody)	ATCC	Cat# TIB-207
53-6.72 (Anti-CD8a antibody)	ATCC	Cat# TIB-105
Experimental models: Organisms/strains
C57BL/6JJmsSlc	Japan SLC	N/A
Oligonucleotides
CpG–ODN (CpG K3: 5′-atcgactctcgagcgttctc-3′)	Gene Design	Cat# CN-65003
Software and algorithms
GraphPad Prism 7.03	GraphPad Software	Version 7.03
Flowjo Software	TreeStar	N/A
Other
Microplate Reader (Power Wave HT)	BioTek	N/A
NovoCyteFlow Cytometer	ACEA Bioscience	N/A
Attune NxT Flow Cytometer	Thermo Fisher Scientific	N/A
Gentle MACS Dissociator	Miltenyi Biotech	N/A
AKTA pure 25 L1	GE Healthcare	N/A