Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 27;106(10):2624–2632. doi: 10.3324/haematol.2020.256313

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2021 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

PMC Copyright notice

Figure 3. — CDK6 alters the composition of the hematopoietic stem and progenitor pool. Analysis was performed 3 weeks post final polyinosinic–polycytidylic acid (poly(I:C)) injection. (A) Gating and representative flow cytometry pseudocolour plots. Percentage of (B) Lin^-Sca-1⁺c-Kit⁺ (LSK) and (C) hematopoietic stem cells (HSC) (CD150⁺CD48^-CD135^-CD34^-LSK) per 2.5x10⁶ bone marrow (BM) cells (Cdk4^Δ/Δ; Cdk6^Δ/Δ; Cdk4/6^fl/fl, n≥5/genotype). (D) Representative gating of DAPI/Ki-67 cell cycle staining of CD150⁺CD48⁺LSK (Fraction B) cells (E) and quantification of % cells in G0 (DAPI^-, Ki-67^-), G1 (DAPI^-, Ki-67⁺) or S/G2/M (DAPI⁺, Ki-67⁺) cell cycle phase per 2.5x10⁶ BM cells (n=3 (Cdk4^Δ/Δ); n=3 (Cdk6^Δ/Δ); n=6 (Cdk4/6^fl/fl)). All comparisons were done with one-way ANOVA followed by Bonferroni’s Multiple Comparison Test, *P≤0.05, **P≤0.01, ***P≤0.001.