Fig. 4.

MiR-200b-3p alleviated TNF-α-induced apoptosis and TNF-α-inhibited proliferation through KHDRBS1. A The expression of miR-200b-3p in each group was detected by qRT-PCR. (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.001; ^###P < 0.001; ^‡‡‡P < 0.001). B qRT-PCR analysis of KHDRBS1 expression in each group (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.001; ^###P < 0.001; ^‡‡‡P < 0.001; ^{^^^}P < 0.001; ^§§§P < 0.001). C Western blot analysis of KHDRBS1 expression in each group. D The signal intensities of KHDRBS1 were quantified. (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.001; ^###P < 0.001; ^‡‡‡P < 0.001; ^{^^^}P < 0.001; ^§§§P < 0.001). E Cells viability was measured by MTT in each group (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.001; ^###P < 0.001; ^‡‡‡P < 0.001; ^{^^^}P < 0.001; ^§§§P < 0.001). F Apoptotic progression was determined by Flow cytometric analysis. G The results were expressed as percentages of positive mean values ± SD (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.001; ^##P < 0.01; ^‡‡‡P < 0.001; ^{^^}P < 0.01; ^§§§P < 0.001). Group: Blank group; TNF-α group; TNF-α+MC group; TNF-α+M group; TNF-α+NC group; TNF-α+KHDRBS1 group; TNF-α+M+KHDRBS1 group. qRT-PCR quantitative reverse transcription polymerase chain reaction, KHDRBS1 KH RNA binding domain containing, signal transduction associated 1, MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, TNF-α tumor necrosis factor-α