Fig. 5.

The effects of miR-200b-3p and KHDRBS1 on apoptosis-related proteins in vitro. A Western blot analysis of C-Caspase-3, Bcl-2 and Bax expression in each group. B–D The signal intensities of C-Caspase, Bcl-2 and Bax were quantified (n = 3, * vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^**P < 0.01; ^***P < 0.001; ^###P < 0.001; ^‡‡P < 0.01; ^‡‡‡P < 0.001; ^{^^^}P < 0.001; ^§§P < 0.01). E and F qRT-PCR analysis of Bcl-2 and Bax expression in each group rats (n = 3, ^* vs. Blank; ^# vs. TNF-α+MC; ^{^} vs. TNF-α+M; ^§ vs. TNF-α+NC; ^‡ vs. TNF-α+KHDRBS1. ^***P < 0.01; ^##P < 0.01; ^###P < 0.001; ^‡‡P < 0.01; ^‡‡‡P < 0.001; ^{^^}P < 0.01). Group: Blank group; TNF-α group; TNF-α+MC group; TNF-α+M group; TNF-α+NC group; TNF-α+KHDRBS1 group; TNF-α+M+KHDRBS1 group. qRT-PCR quantitative reverse transcription polymerase chain reaction, KHDRBS1 KH RNA binding domain containing, signal transduction associated 1, TNF-α tumor necrosis factor-α, Bcl-2 B cell lymphoma/lewkmia-2, Bax Bcl-2 associated X protein