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. Author manuscript; available in PMC: 2021 Oct 6.
Published in final edited form as: Chromosoma. 2019 Jun 29;128(3):369–383. doi: 10.1007/s00412-019-00713-9

Fig. 5. Absence of Sirt7 does not affect RPA or DMC1 foci formation but affects the number of cross over events.

Fig. 5

(a, d) Representative confocal images of zygotene and pachytene-staged oocytes immunostained with antibodies to detect SYCP3 (red) and RPA (gray) (a) or DMC1 (d) (gray) from wild-type (WT) and Sirt7−/− (KO) females at embryonic day 16.5. (b-c) Quantification of the number of RPA foci per oocyte at zygonema (b) (n=3 experiments, WT: 61, KO 52 oocytes) and pachynema (c) (n=3 experiments, WT: 51, KO 48 oocytes). (e-f) Quantification of the number of DMC1 foci per oocyte at zygonema (n=3 experiments, WT: 32, KO 23 oocytes) (e) and at pachynema (n=3 experiments, WT: 53, KO 51 oocytes) (f). (g) Representative confocal images of pachytene-staged oocytes immunostained with antibodies to detect SYCP3 (red) and MLH1 (gray) from WT and KO females at embryonic day 18.5. (h) Quantification of the number of MLH1 foci per oocyte at pachynema (n=3 experiments, WT: 63, KO 62 oocytes). Graph shows individuals values plus the mean ±SEM from 3 independent experiments. *** P < 0.001; scale bars, 10μm.