Figure 3.

NAFLD patients present impaired autophagy with increased TXNIP expression. (A) Representative images of immunohistochemical staining for MAP1LC3B and SQSTM1 in liver sections from controls (n = 4) and patients with NAFLD (n = 11). Scale bars: 100 μm, 50 μm, and 5 μm, respectively. The numbers of MAP1LC3B puncta and semiquantitative analysis of the IOD of SQSTM1-positive areas are shown below the image. Ten fields (final magnification, × 400) were randomly selected for each sample, and the positive staining in each image was measured. (B) Correlation between TXNIP and MAP1LC3B or SQSTM1 expression in human NAFLD patients. Values represent means ± SEM. *P < 0.05, **P < 0.01 versus control