Figure 1.

Epidermal autophagy deficiency inhibits wound healing. (A) Macroscopy view of wound healing on days 0, 2, 4 and 6 in WT, atg5 cKO, and atg7 cKO mice. Scale bar: 1 mm. (B) Quantification of A. (C and E) Wound healing as monitored by histological staining of skin sections at the wound edges at 2, 4, 6 and 8 d after injury in WT, atg5 cKO and atg7 cKO mice. Epi: Epidermis; Der: Dermis; Es: Eschar. GT: Granulation Tissue. Black dotted lines denote dermal-epidermal boundaries (C). Scale bar: 200 μm. (D) Quantification of the length of hyper-proliferative epidermis. (F) Quantification of the granulation tissue areas. All bars represent mean ± SD (n = 6). *, P < 0.05; *, P < 0.01; ***, P < 0.001; compared with WT mice (B) or between comparison groups (D, F); Student’s t-test