Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 6;40(40):5913–5924. doi: 10.1038/s41388-021-01987-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a m6A peaks were enriched in the CDS and 3′ UTR of the DEGS2 according to the m6A-RIP-seq data. Squares indicate marked decreases in m6A peaks in CRC tissues compared to control tissues; b m6A-RIP assay confirmed the exact m6A location in the CDS region of DEGS2 mRNA. GAPDH mRNA was used as a nontarget control, and MYC mRNA was used as a positive control. ns, not significant; *p < 0.05. c Schematic representation of the position of the m6A motifs within DEGS2 mRNA. d Schematic representation of the mutation (GACT to GGCT) in the CDS to investigate the roles of m6A in DEGS2 expression. e sh-METTL3, LV-DEGS2-mut, and LV-DEGS2-WT were transfected into HCT116 cells for 24 h. Protein expression was measured by WB (bottom panel) and quantitatively analyzed (upper panel). f knockdown of YTHDF2 promoted the DEGS2 mRNA level. g knockdown of METTL3 reduced m6A methylation of DEGS2.