Background and aims
To study the effect of heating at 56 °C for 30 min on the monitoring of five second-generation antipsychotics (aripiprazole, quetiapine, risperidone, clozapine and olanzapine).
Methods
After the plasma samples were treated with protein precipitation, the concentrations of aripiprazole, quetiapine, risperidone, clozapine and olanzapine were determined by LC-MS method before and after inactivation of plasma quality control substances and clinical samples. Bland-Ahman method was used to evaluate the difference between the inactivated and non-inactivated determination results, and the correlation between the determination results was analyzed by Passing-Bablok regression and linear regression.
Results
The five second-generation antipsychotics could remain stable after inactivation, and there was no significant difference in matrix effect before and after inactivation of serum quality control substances, and the concentration percentages before and after inactivation were all within the normal range.
Conclusions
The combination of 56 °C for 30 min inactivated protein precipitation treatment method of plasma samples of LC - MS determination o made pp, quetiapine, risperidone, clozapine, olanzapine plasma concentration is affected, the determination results have good consistency and relevance, o can be used to made pp, quetiapine, risperidone, clozapine, olanzapine routine therapeutic drug monitoring.