Corrigendum to: Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis

Plant Cell (2010) 22: 143–158. doi: 10.1105/tpc.109.068320.

The authors have discovered that an error occurred in the assembly of Supplemental Figure 6 panels B and C where it appears that loading controls were reused from Supplemental Figure 1.

The primary data and materials relevant to this work are no longer available. A.R. retrieved independent digital scans for 8 immunoblots that were either included (3) or discarded (5) during the assembly of Supplemental Figure 6B and C; however these scans do not provide sufficient information to assign the corresponding loading controls with confidence. Nevertheless, we stand by the results presented in Supplemental Figure 6B and C for the following reasons:

1. Supplemental Figure 6B and 6C show a negative result (similar protein accumulation between wild-type and rpl4a mutant). This is consistent with the mild phenotypes observed in the adult rpl4a mutant (Figure 2E), and the presence of compensatory mechanisms between RPL4 proteins (Figure 5).

2. Finding a positive result (differences in protein accumulation between wild-type and the rpl4a mutant) would have provided broader mechanistic insight to the function of the RPL4A protein. We performed multiple immunoblots using different antibodies (e.g. Chitinase, Lectin, MV39, SYP61, VPS45, VTI12), and despite these efforts, the results obtained were reproducible and consistently negative.

3. The immunoblot scans retrieved by A.R. are time-stamped with three different dates. This makes the possibility of a pervasive experimental error with differential protein loading between wild-type and rpl4a lanes across multiple blots highly unlikely.

We would also like to state that the results presented in Supplemental Figures 6 panels B and C do not affect, change, or interfere with any of the other findings and conclusions presented in the manuscript. We greatly regret this oversight, which is believed to be the result of an inadvertent error in the assembly of these composite figures due to the similarity of the gel crops and images of the different loading controls.

Editors’ note: This correction notice was reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.