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. 2021 Oct 8;8(5):ENEURO.0062-21.2021. doi: 10.1523/ENEURO.0062-21.2021

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Copyright © 2021 Brown et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — A, Representative photomicrographs of NeuroD⁺ NPs in E19 dentate anlage. IUGR offspring of both sexes had increased percentages of NeuroD⁺ NPs (number of NeuroD⁺ cells divided by number of DAPI⁺ cells) in dentate anlage (p = 0.015, IUGR males and females compared with sex-matched sham controls). B, Representative photomicrographs of Prox1⁺ immature and mature glutamatergic granule neurons in E19 dentate anlage. IUGR offspring of both sexes had increased percentages of Prox1⁺ granule neurons (the number of Prox1⁺ cells divided by the number of DAPI⁺ cells) in dentate plate and granule cell layers of the future suprapyramidal and infrapyramidal blades (p = 0.009 and p = 0.008, respectively in IUGR males and females compared with sex-matched sham controls). Two-way ANOVA with Tukey’s HSD post hoc test was used to examine the effects of treatment (sham or IUGR), sex, or both sex and treatment on the percentages of NeuroD⁺ or Prox1⁺ cells.