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PrKD1 phosphorylates CDC25 phosphatase. A, In vitro phosphorylation assay showed that PrKD1 phosphorylates CDC25 phosphatases at their common phosphorylation site: CDC25 A, CDC25B and CDC25C. B, Western blot analysis for CDC25C phosphorylation at serine 216 with reference to PrKD1 and activation of C4-2. γ-Radiation (γ-rad) was performed for DNA damage induction. Bryostatin (bryo-1) treatment was used for activation of exogenous PrKD1 after induction. PrKD1: protein kinase D1
