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. 2021 Sep 30;184(20):5201–5214.e12. doi: 10.1016/j.cell.2021.08.029
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-SAP05 antibody this study RRID:AB_2893243
Anti-HA tag antibody Eurogentec Cat# MMS-101R; RRID:AB_291262
Anti-GFP antibody Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695
Anti-FLAG antibody Sigma Cat# F-3165; RRID:AB_259529
Penta·His Antibody QIAGEN Cat# 34660; RRID:AB_2619735
Anti-AtRPN10 Agrisera Cat# AS194266; RRID:AB_2893242

Bacterial and virus strains

Aster yellows witches’-broom phytoplasma this study Taxonomy ID: 322098
Escherichia coli BL21(DE3) Novagen Cat# 70235
Agrobacterium tumefaciens GV3101 N/A N/A

Chemicals, peptides, and recombinant proteins

Human 26S Proteasome R&D Systems Cat# E-365-025
MG132 Sigma Cat# M7449
Bortezomib Sigma Cat# 5043140001
E-64d Sigma Cat# E8640
3-MA Sigma Cat# M9281
GFP-Trap Magnetic Agarose ChromoTek Cat# gtma-20

Experimental models: Organisms/strains

Yeast strain AH109 N/A N/A
Yeast strain NMY51 N/A N/A
Arabidopsis thaliana Col-0 N/A N/A
Arabidopsis thaliana overexpressor line p35S::GFP this study N/A
Arabidopsis thaliana overexpressor line p35S::SAP05_AY-WB this study N/A
Arabidopsis thaliana overexpressor line pAtSUC2::GFP this study N/A
Arabidopsis thaliana overexpressor line pAtSUC2::SAP05 this study N/A
Arabidopsis thaliana overexpressor line pSPL13::rSPL13-GUS NASC NASC ID: N69817
Arabidopsis thaliana overexpressor line pSPL11::rSPL11-GUS NASC NASC ID: N69815
Arabidopsis thaliana mutant line rpn10-2 NASC NASC ID: N366730
Arabidopsis thaliana overexpressor line p35S::MIR156A NASC NASC ID: N67849
Arabidopsis thaliana mutant line spl9 spl11 spl13 spl15 NASC NASC ID: N69797
Arabidopsis thaliana overexpressor line p35S::MIM156 NASC NASC ID: N9953
Arabidopsis thaliana mutant line gnc gnl gata17 gata17l Ranftl et al., 2016 N/A
Arabidopsis thaliana overexpressor line p35S::GNC Ranftl et al., 2016 N/A
Arabidopsis thaliana overexpressor line p35S::SAP05_WBDLa this study N/A
Arabidopsis thaliana overexpressor line p35S::SAP05_WBDLb this study N/A
Arabidopsis thaliana overexpressor line p35S::SAP05_PnWBa this study N/A
Arabidopsis thaliana overexpressor line p35S::SAP05_PnWBb this study N/A
Arabidopsis thaliana rpn10-2 mutant wild-type allele complementation line cRPN10 this study N/A
Arabidopsis thaliana rpn10-2 mutant engineered m1 allele complementation line eRPN10 this study N/A
Arabidopsis thaliana cRPN10 X p35S::SAP05 line this study N/A
Arabidopsis thaliana eRPN10 X p35S::SAP05 line this study N/A
Arabidopsis thaliana mutant line han-2 Ding et al., 2015 N/A
Arabidopsis thaliana han-2 X p35S::CsHAN Ding et al., 2015 N/A

Recombinant DNA

pB7WG2 (gene specific constructs listed in Table S5) VIB-UGent Center for Plant Systems Biology Vector ID: 1_04
pHW59 (gene specific constructs listed in Table S5) Mathieu et al., 2007 N/A
pEarleyGate202 (gene specific constructs listed in Table S5) Tair Stock# CD3-688
pB7WGF2 (gene specific constructs listed in Table S5) VIB-UGent Center for Plant Systems Biology Vector ID: 1_42
pB7RWG2 (gene specific constructs listed in Table S5) VIB-UGent Center for Plant Systems Biology Vector ID: 1_63
pBI121 (gene specific constructs listed in Table S5) Tair Stock# CD3-388
pGADT7 (gene specific constructs listed in Table S5) Addgene Plasmid #61702
pGBKT7 (gene specific constructs listed in Table S5) Addgene Plasmid #61703
pGADHA (gene specific constructs listed in Table S5) Dualsystems Biotech N/A
pLEXA-C (gene specific constructs listed in Table S5) Dualsystems Biotech N/A
pUGW15 (gene specific constructs listed in Table S5) Pecher et al., 2019 N/A
pOPINF (gene specific constructs listed in Table S5) Berrow et al., 2007 N/A
pOPINS3C (gene specific constructs listed in Table S5) Berrow et al., 2007 N/A
pOPINA (gene specific constructs listed in Table S5) Berrow et al., 2007 N/A

Software and algorithms

Prism 7 Graphpad https://www.graphpad.com/scientific-software/prism/
Fiji ImageJ https://imagej.net/software/fiji/
Phylogeny.fr (Dereeper et al., 2008) http://www.phylogeny.fr/index.cgi