Figure 6.

Measurement of autophagic flux in different storage and collection conditions. (A) Whole blood was taken from subjects and stored at room temperature for four h and then processed for measurement of autophagic flux by western blot for LC3B (2 subjects displayed). (B) ΔLC3B-II normalized to ACTB was determined for blood taken from three subjects and processed for autophagic flux after storage at room temperature (RT) for 0 h or 4 h. (C) Whole blood was taken from subjects and stored on ice for four h and then processed for measurement of autophagic flux by western blot for LC3B (2 subjects displayed). (D) ΔLC3B-II normalized to ACTB was determined for blood taken from three subjects and processed for autophagic flux after storage on ice for 0 h or 4 h. (E) Whole blood was collected from subjects in different tubes – lithium heparin- (Li-Hep) or EDTA-containing tubes were tested. Blood was then processed for analysis of autophagic flux by western blot for LC3B (2 subjects displayed). (F). ΔLC3B-II normalized to ACTB was determined for blood taken from three subjects using lithium heparin- (Li-Hep) or EDTA-containing tubes. S: subject. Mr: molecular weight marker