Figure 3. Correlation between individual transcriptomes and behavioral biases.

(A) Steps for collecting transcriptomes from flies that have completed the Decathlon. (B) Data matrix of individual head transcriptomes. Rows are individual flies (n = 98). Columns are 17,470 genes sorted by their mean expression across individuals. The dashed line indicates the mean expression cutoff at 10 reads per million (RPM), below which genes were excluded from analysis. (C) Scree plots of the logged % variance explained for ranked principal components of gene expression variation, for observed (red) and shuffled (black) data. Shaded region corresponds to 95% CI as calculated by bootstrap resampling. (D) Performance heatmap (-log p) of linear models predicting the behavior of individual flies from single-gene expression. Colored bars (left) indicate a priori group identity of behavioral measures (rows). Bar graphs show the number of significant (p<0.05) models for each gene (top) and behavior (right). (E) Heatmap showing the probability across bootstrap replicates of a KEGG pathway being significantly enriched in the list of predictive genes for a given behavior. (F) Bar plot showing the average across bootstrap replicates of the maximum (across behaviors) negative log adjusted p-value of all enriched KEGG pathways. Color indicates results from observed (gray) or shuffled control (black) data. (G) Average maximum adjusted -log p-value for enriched KEGG pathways common to all Decathlon iterations. Pathway labels (right) are ordered by batch 3 (outbred) -log adjusted p-value.

Figure 3—figure supplement 1. Effect of thermogenetic neural perturbation on clumpiness and switchiness.

(A) Schematic of the experiment for testing the effects of neural perturbation on switchiness and clumpiness. Plot depicts the schema used to activate or silence neurons. (B) Diagram of the various neurons (solid lines) targeted by lines tested in the screen. See Supplementary file 3 for list. (C) Example turn data from individuals exhibiting low and high clumpiness (upper rows) and low and high switchiness (bottom rows). (D) Scatter plots of line average clumpiness and switchiness at the permissive (left) and restrictive (right) temperatures. Lines indicate the line of best fit and shaded regions indicate the 95% confidence of the fit as determined by bootstrap resampling. (E) Average correlation coefficients for each effector type at the permissive and restrictive temperatures. Error bars indicate the 95% confidence interval as determined by bootstrap resampling. Panels (A) and (B) have been reproduced from Figure 2B and G of Skutt-Kakaria et al., 2019 Bioxiv, published under a CC BY 4.0 license.