Figure S5.

Effects of the LRRK2 G2019S mutation on mitochondrial fitness, related to Figure 5

(A) Schematic drawing of the Agilent Seahorse XF Cell Mito Stress Test profile, showing key parameters of mitochondrial function upon inhibition of the Electron Transport Chain complexes.

(B) Oxygen Consumption Rate (OCR) of WT microglia (gray) and microglia carrying the LRRK2 G2019S mutation (blue) under basal conditions. n = 3 independent experiments.

(C) Oxygen Consumption Rate (OCR) of WT microglia (gray/black) and microglia carrying the LRRK2 G2019S mutation (light blue/dark blue) upon treatment with 2 μM fibrillar α-syn for 24 h. n = 3 independent experiments.

(D) Representative immunocytochemical staining and 3D reconstructions of WT microglia and microglia carrying the LRRK2 G2019S mutation demonstrating increased mitochondrial circulation (TOM20, green) following exposure to fibrillar α-syn (orange).

(E–G) Quantification of the mitochondrial length (E), elongation score (F), and the mitochondrial area per cell size (G) of WT and LRRK2 G2019S mutant microglia. n = 3 independent experiments.

(H) Quantification of the mean ROS release of WT microglia and microglia carrying the G2019S mutation under basal conditions and upon treatment with 2 μM α-syn fibrils for 24 h. n = 6 for WT and n = 4 for LRRK2 G2019S.

All graphs are presented as mean ± SEM and were analyzed by two-way ANOVA. ^∗∗∗∗p < 0.0001, ^∗∗∗p < 0.001, ^∗∗p < 0.01; ^∗p < 0.05.

Scale bar: 10 μm.