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. 2021 Sep 30;11(10):1437. doi: 10.3390/biom11101437

Figure 4.

Figure 4

Syncytium cell formation and cell viability of HIV-1-infected SupT1 cells expressing ankyrins. (A) SupT1 cells and SupT1 stable cells were infected with HIV-1 NL4-3 laboratory strain at 10 MOI. Infected cells were cultured and processed as shown. After HIV-1 challenge, cells were subcultured every 2 days. (B) At 13 days post-infection, syncytium formation in infected cells was observed under microscopy. Cell imaging was done at 20× magnification using Axio Vert.A1. Arrows point to syncytium cells. (C) Syncytium formation in infected SupT1/Myr (+) AnkGAG1D4-EGFP and SupT1/Myr (+) AnkGAG1D4-S45Y-EGFP was continuously observed until 21 days post-infection. (D) Cell viability of infected cells was determined using the Trypan blue exclusion method. No ankyrin, AnkA32D3, AnkGAG1D4, and AnkGAG1D4-S45Y represent SupT1 cell control, SupT1 cells expressing Myr (+) AnkA32D3-EGFP, Myr (+) AnkGAG1D4-EGFP, and Myr (+) AnkGAG1D4-S45Y-EGFP, respectively.