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. 2021 Oct 15;11(10):1522. doi: 10.3390/biom11101522

Figure 11.

Figure 11

Reoxygenation inhibits cell proliferation and induces IL-6 production in an IDO dependent manner. Cells were cultured under anoxic conditions for 24 h and then subjected to 2 h of reoxygenation along with the indicating treatments. Reoxygenation reduced the levels of the cell proliferation marker Ki-67. The IDO inhibitor 1-MT restored Ki-67 levels, whereas the AhR inhibitor CH223191 did not affect Ki-67. A representative Western blot experiment is displayed in panel (A), whereas panel (B) depicts the cumulative results of four repeated experiments. Reoxygenation increased IL-6 concentration in the RPTECs culture supernatants. 1-MT reduced the reoxygenation-induced IL-6 production, whereas CH223191 did not affect IL-6 levels (C). Six such experiments were performed. * p < 0.05 vs. Control, # p < 0.05 vs. Control with 1-MT, ^ p < 0.05 vs. Control with CH223191, & p < 0.05 vs. Reox, + p < 0.05 vs. Reox with 1-MT, ! p < 0.05 vs. Reox with CH223191. Error bars correspond to SEM. 1-MT, 1-DL-methyltryptophan; IL-6, interleukin-6; Reox, reoxygenation.