Table 2.

Near full-length single-genome amplification primers.

Pre-Nested Primers
Primer Name	Primer Direction	Nucleotide Position in HXB2 (bp)	5′-3′ Sequence
Li_OuterF +,*	Forward	623–649	AAATCTCTAGCAGTGGCGCCCGAACAG
Li_OuterR	Reverse	9662–9686	TGAGGGATCTCTAGTTACCAGAGTC
Nested Primers
Primer Name	Primer Direction	Nucleotide Position in HXB2 (bp)	5′-3′ Sequence
Li_InnerF *	Forward	769–793	GCGGAGGCTAGAAGGAGAGAGATGG
Li_InnerR +	Reverse	9604–9632	GCACTCAAGGCAAGCTTTATTGAGGCTTA
NFL-alt_in_F #	Forward	642–664	CCG AAC AGG GAC BHG AAA GCG AA

⁺ Salminen et al., 1995, * Li et al., 2010, ^# Katusiime et al., 2020.