Figure 3.

Oncogenic KRAS drives aberrant nuclear morphology in vitro and in vivo. (A) 4292F murine PDAC cells with a doxycycline (Dox)-inducible KRAS^G12D are shown, lamin B1 (green), DAPI (blue). Cells that are not expressing oncogenic KRAS (-Dox) present large and rather uniform nuclei. Induction of oncogenic KRAS with Dox causes a significant reduction in nuclear size and presents lamina alterations. Bar = 10 µm. (B) A 3D reconstruction from Z-stacks of DAPI signal from 4292F PDAC cells grown without or with Dox. All images were sized proportionally. (C) Murine H&E tissue sections from normal pancreas of Cre mice and PDAC pancreas from KRAS^G12D mice (KC). Normal pancreas displays larger and more uniform nuclei than those from PDAC. Bar = 100 µm. Inset regions are indicated by black rectangles and shown at higher magnification at right. Inset bar = 50 µm.