FIG 2.

Enzymatic activity of the enzymes of the lower glycolytic pathway of the D457 parental strain and the fosfomycin-resistant mutants. (A) Gap, glyceraldehyde-3P dehydrogenase activity. (B) Pgk, phosphoglycerate kinase activity. (C) Gpm, phosphoglycerate mutase activity. (D) Eno, enolase activity. Error bars indicate standard deviations for the results from three independent replicates. As shown, each of the mutants exhibits an impaired activity of the enzyme encoded by the mutated gene. Values that are significantly different from the value for the wild-type D457 strain by a unpaired two-tail t test are indicated by asterisks as follows: *, P < 0.02; **, P < 0.002; ****, P < 0.0001.