Table 1.

Overview of techniques, resources, tools, and lines available for functional genomics studies in Nematostella. Updated and extended from [19].

Culture and Experimentation
Rearing and spawning	[28,31,32]
Inducing and assessing regeneration	[33,34,35]
Microinjection and micromanipulation	[36,37,38,39,40,41,42,43,44,45,46,47]
Genome, Resources, and Protocols for -Omics Analyses
Annotated genome—V1.0	[18] https://mycocosm.jgi.doe.gov/Nemve1/Nemve1.home.html (accessed on 5 October 2021)
Annotated genome—V2.0	[48] https://genomes.stowers.org/starletseaanemone (accessed on 5 October 2021)
Microarrays	[49,50,51,52]
RNA-seq/transcriptomes	[20,53,54] http://nvertx.kahikai.org (accessed on 5 October 2021)
scRNA-seq	[55]
ATAC-seq	[55]
ChIP-seq	[56]
Spatio–Temporal Gene Expression Analysis
mRNA in situ	[57,58,59,60,61,62,63,64]
Immunohistochemistry	[36,37,39,40,46,65,66,67]
Transgenic reporter	[68,69,70,71,72,73]
Tools to Study Gene Function
mRNA over-expression	[40,47,49,51,65]
Morpholino	[36,39,41,46,47,49,51,63,65]
Short hairpin RNA	[74,75]
TALEN/Fok1, CRISPR/Cas9	[69]
Heat-shock inducible promoters	[69]
Pharmaceutical treatments	[21,39,51,71,76,77,78,79,80]
Available Stable Reporter Lines
NvMyHC::mCherry	[68]
NvElav::mOrange	[81]
NvElav::Cerulean	[82]
NvLWamide:mCherry	[73]
NvEf1a::mOrange-CAAX	[83]
NvFoxQ2d::mOrange-CAAX	[82]
NvNcol-3::memOrange2	[84]
NvAnthox8::Gfp	[74]
NvPou4::mCherry	[70]
NvTBP::mCherry	[85]
Available Stable KO Lines
NvAnthox1a−/−	[74]
NvAnthox6−/−	[74]
NvAnthox8a−/−	[74]
NvFgfrB+/−	[71]
NvPou4+/−	[70]