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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
The underlying obstacles and corresponding solutions for efficient CRISPR system delivery in the form of plasmid, RNA (Cas9 mRNA, sgRNA), and Cas9 RNP (Created with BioRender.com, accessed on 30 September 2021).