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. 2021 Oct 15;26(20):6246. doi: 10.3390/molecules26206246

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The %RSD comparison for fatty acid derivatization methods. Plot comparison of all %RSD values for each derivatization technique and each biological matrix. The red line across the plot at 15 represents a commonly accepted cutoff for highly reproducible metabolomics data. (A) The %RSD values for the 33 fatty acid standards utilized to validate the method. (B) The reproducibility of each fatty acid in the following biological matrices: human prostate cancer cell line DU145; fetal bovine serum (FBS); human liver cancer cell line HepG2; and U937, a human myeloid leukemia cell line.