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Tail spike expression and confirmation of activity. (a) SDS-PAGE gel of E. coli KRX cells expressing the recombinant tail spike protein. Lanes: SeeBlue Plus2 reference ladder (L), total protein (1), soluble protein fraction (2). A band of approximately 72 kDa, corresponding to a monomer of the tail spike protein, is visible (indicated with a red arrow). (b) Spot testing (10 µL) of the purified tail spike protein and phage suspension on a lawn of P. mirabilis BB2000.
