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Transgene cas9 and mutation screening in T1 generation rice plants. (A) Transgene screening of eIF4G-targeted T1 transgenic plants by genomic DNA PCR. WT: wild-type plants. M: DNA marker. (B) eif4g mutant screening in eIF4G-targeted T1 transgenic plants (#4) by genomic DNA PCR. WT: wild-type plants; DNA sequence deletions are denoted by black dashes, and DNA sequence insertions are denoted by blue letters. The sgRNA target sequences are shown in red letters. M: DNA marker.
