Figure 4.

Label-free identification of dNMPs using the dual-nanopore TOF sensor. (a-c) Histograms for the normalized counts of TOF obtained with four dNMPs using a nanosensor with 0.5, 1, and 5 μm-long nanochannel column, respectively, and the corresponding Gaussian fits. The insets show the identification accuracies of dNMPs, where each value was obtained by the ratio of the non-overlapped area to the entire area of the Gaussian curve fit for a histogram. (d) Average identification accuracy. The fitting curve with IA (%) = 100[1 − exp(−1.2998 · l)] was included, where IA (%) is the identification accuracy in % and l is the nanochannel column length. (e) Apparent mobility versus the length of the nanochannel column. Data were acquired with a 1 m KCl solution containing 0.5× TBE, pH 10.0 under a driving voltage of 3 V. The nucleotide concentration in cis-compartment was 0.01 ng·μL⁻¹.