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Comparison of the ancestral plasmid pUR2940 and its evolved variants. The ancestral plasmid pUR2940t0, evolved plasmid pUR2940t35 from MW2 evolved clones 1, 2, and 3, and evolved plasmid pUR2940t14 isolated from evolved strain C2940 are compared. Image visualization was carried out with BLAST Ring Image Generator (BRIG), which shows the similarity between the central reference sequence pUR2940t0 and the other variants as concentric rings. ORFs, open reading frames; CDS, coding sequence.
