TABLE 1.
Characteristics of plasmids used to test the curing efficiency of pEMPTY::sgRNA2
| Plasmid name | Origin | Size (pb) | GenBank no. | gRNA2 target sequence | Resistance determinant(s) | Toxin-antitoxin system | Encoded bacteriocin |
|---|---|---|---|---|---|---|---|
| pMW2 | S. aureus MW2 (CA-MRSA); clonal lineage USA400; CC1 (17) | 20654 | NC_005011.1 | Yes | cadDX, blaIRZ | Type I, Fst family | Lactococcin |
| pUR2940 | S. aureus C2940 (LA-MRSA); ST398; CC398 (18) | 23702 | MW367469 | Yes | cadDX, erm(C), erm(T), dfrK, tet(L), copA | None | None |
| pN315 | S. aureus N315 (HA-MRSA); clonal lineage USA100; CC5 (19) | 24653 | NC_003140.1 | Yes | cadDX, blaIRZ, arsBC | Type I, Fst family | Lactococcin |
| pLAC-p03 | S. aureus LAC (CA-MRSA); clonal lineage USA300; CC8 (20, 50) | 27068 | NZ_CP002149.1 | Yes | cadDX, msrA, mphC, blaIRZ, aph(3′)-IIIa | Type I, Fst family | None |
| pUR1902 | S. aureus C1902 (LA-MRSA); ST398; CC398 (18) | ≈2000 | HF583291.1 (partial sequence) | Yes | copA, mco, tet(L), cadDX, erm(T), aadD | None in the available sequence | None in the available sequence |
| pUR3912 | S. aureus C3912 (LA-MSSA); ST398; CC398 (51, 52) | 6176 | HE805623.2 | Yes | cadDX, erm(T) | None | None |
| pUR1841 (pLNU1) | S. aureus C1841 (LA-MRSA); clonal lineage ST398; CC398 (53) | 2361 | AM184099.1 | Yes | lnu(A) | None | None |
| pUR2355 | S. aureus C2355 (LA-MRSA); clonal lineage ST398; CC398 (54) | 7609 | JQ312422.1 | No | vga(A) | None | None |