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. 2021 Feb 16;12(1):e03274-20. doi: 10.1128/mBio.03274-20

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FIG 3 — BPV1 purification and bovine organotypic raft control. (A) A total of 200 μl fractions were collected from the bottom of the BPV OptiPrep gradient. Each fraction was separated by SDS-PAGE and stained for total protein with Sypro Ruby (top) and immunoblotted for BPV L1 (middle) or histone H3 (bottom). Blots are representative of 15 independent experiments. (B) Quantification of immunoblots in panel A. (C) Reverse transcriptase qPCR (RT-qPCR) quantification of BPV1 DNA per fraction (fg). (D) Representative image of transmission electron microscopy of isolated BPV virions. (E) Organotypic raft culture generated from bovine keratinocytes as a cellular control.