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. 2021 Feb 23;12(1):e03537-20. doi: 10.1128/mBio.03537-20

FIG 5.

FIG 5

Vaccine peptide-specific serum IgG titers induced in Sprague-Dawley rats after vaccination with P*17/K4S2(CRM). Sprague-Dawley rats were administered an i.m. inoculation of 0.1 mg/dose of P*17/K4S2(CRM)/CAF®01 on days 0 and 21 and an i.n. inoculation of 0.1 mg/dose of P*17/K4S2(CRM)/Tris on day 42. Rats were euthanized at day 46 and day 70. Peptide-specific serum IgG titers (Geomean) are shown for individual rats euthanized at day 46 (n = 10; 5 male, 5 female) and 70 (n = 10; 5 male, 5 female). Pre-vaccination (day −1) serum IgG titers of the same rats assessed at day 46 or 70 are also shown. The endpoint titer was defined as the highest dilution that gave an absorbance of >3 standard deviations above the mean absorbance of negative control wells. Statistical analysis was performed using a nonparametric, unpaired Mann-Whitney U test (one-tailed) to compare groups (ns, P > 0.05; ****, P < 0.0001). Analysis was performed and the graph generated in GraphPad Prism 8.1.2.