FIG 4.

Plasma samples from C. gattii patients tested for the presence of GM-CSF antibodies. (A) The immune status of 32 patients determined prior to (1) and after (2) the plasma test for the presence of GM-CSF autoantibody. (B) The functionality test of anti-GM-CSF autoantibody. Peripheral blood mononuclear cells (PBMCs) were incubated with 10% plasma from healthy volunteers (control) or GM-CSF antibody-positive patients. Cells were then unstimulated or stimulated with 1 ng/ml or 10 ng/ml GM-CSF. pSTAT5 production in PBMCs was analyzed by flow cytometry. Plasma from two patients, R104 and DW-1, tested for the presence of functional GM-CSF antibody are shown as examples.