FIG 5.
Cells producing functional BP3410 protein export manganese. Three biological replicates of wt, ΔBP3410, Mn resister, and Mn resisterC strains were cultured either in the absence (gray symbols) or in the presence (yellow symbols) of 100 μM Mn for 8 h. The amount of intracellular Mn was measured by ICP–MS/MS. The dot plot shows individual data points for all strains, and the horizontal black lines represent mean values. Statistical analysis was performed using an unpaired one-tailed t test; ****, P < 0.00005. ODU, optical density units.