FIG 8.

MOV10 interaction with N proteins from other human CoVs. (A) In all cases, cells were mock infected or infected with the corresponding CoV at an MOI of 0.1: DBT-ACE2 cells infected with SARS-CoV (left), Calu3 2B4 cells infected with SARS-CoV-2 (middle), and Huh-7 cells infected with HCoV-229E (right). At 20 hpi, cell lysates were obtained and immunoprecipitated with anti-MOV10 antibody. MOV10 and N proteins were then detected by Western blotting using specific antibodies. Huh-7 and MOV10-KO cells were infected with SARS-CoV-2, and both N protein accumulation (B) and viral titers (C) were analyzed at the indicated time points. Huh-7 and MOV10-KO cells were infected with HCoV-229E, and both N protein accumulation (D) and viral titers (E) were analyzed at the indicated time points. GAPDH was used as a cytoplasmic marker in all cases. Numbers under the blots indicate the estimated levels of N protein, normalized by GAPDH levels and relative to Huh-7 cells at 18 hpi. The values are means from three independent infections; error bars represent SD. ***, P < 0.001.