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. 2021 Mar 24;6(2):e00013-21. doi: 10.1128/mSphere.00013-21

TABLE 2.

Primer sequences used in quantification

Species Gene of interest Sequences (5′ to 3′)
B. dorei topI Forward: AAGCGGCTTCAAGAAACAGG; reverse: GTGCCCTTTACCTTGGGAAC
B. fragilis gyrA Forward: GTGCCCTTCCCGATGTTAGA; reverse: TCCGTGCGGGTGATACTTAC
B. ovatus topI Forward: GGGCCTATTATCGCAACCGA; reverse: AGGTGCATACGTAGACGGAC
B. thetaiotaomicron topI Forward: GTCTGTAATCAAGTCCGCCG; reverse: AATGCCGGAAAGCGGTAAAC
B. adolescentis topI Forward: CTCCGGATACACGGTCATGG; reverse: GTCTTCGATATCCACGCCGA
B. hansenii gyrA Forward: GACGTAAGAAGCACCGGTAGA; reverse: ATAATCGCCCTGACAGGTAAGC
C. difficile gyrA Forward: GGTTGAAAGAATAGCAGAGTTAGTT; reverse: GCATTAGCATCCCTCTTTAATTCTA
E. coli gyrA Forward: GAACTCGGTGAGGACGGTTT; reverse: GCTGGAACAGGACGAACGTA
E. hallii gyrA Forward: TACCGCCTCATCGGACTTGA; reverse: TCATGGAGGCTGGATGCTCT
F. prausnitzii gyrA Forward: CCGGTGTCCGTGTCATGC; reverse: CTCAGCCTCTACTGTCTCGG
R. gnavus gyrA Forward: GCTGAACAGAGCAGAAGAGC; reverse: TCCTTCGCAGTCTGAACATTCT