P cycle-mediated NO reduction in nitrite-dependent NO biosynthesis. (A to C) qRT-PCR for gene expression of the P cycle (A), cytb1 complex (B), and NO-dependent NO biosynthesis (C). (D) Outline for changes in gene expression of NO biosynthesis based on data in panel A. Blue, genes detected; gray, genes undetected. (E) Activity of enzyme activity in the P cycle. (F) NADH level. (G) Nitrite level. Results in panels A to C and E to G are displayed as means ± SEM, and significant differences as determined by two-tailed Student’s t test are identified (**, P < 0.01).