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. 2021 Oct 9;24(11):103250. doi: 10.1016/j.isci.2021.103250

Figure 4.

Figure 4

Deletion of IL-1beta increases beta cell mass and insulin secretion in isolated islets

(A–D) GSIS of isolated islets of 52-weeks-old IL-1beta ko (red) and littermate WT control (black) mice; (A) 1 h insulin secretion expressed as percentage of the insulin content per mouse; (B) insulin content per islet; (C) insulin fold stimulation by glucose, (mean of 4 x 10 islets per mouse); (D) number of islets isolated per mouse; n = 11–12 WT and n = 13–14 IL-1beta ko mice.

(E) Example of immunohistochemical staining of pancreata from IL-1beta ko and WT control mice (green = insulin, blue = DAPI, scale bar, 400 μm).

(F and G) Beta cell mass and mean islet area from 3–4 sections/mouse of 24- and 52-week-old WT and IL-1beta ko mice.

(H) Number of islets per pancreas section.

(I) Frequency distribution of islet area from 52-week-old IL-1beta ko mice and WT mice.

(J) Percentage of islets with one or more Ki67+/ins+/DAPI + cell(s); 472 islets from 4 IL-1beta ko mice and 509 islets from 6 littermate control mice were analyzed.

(K) Ki67+/ins+/DAPI + normalized to the mean ins + islet area.

(L, N–P) Relative gene expression of 52-week-old IL-1beta ko versus littermate WT control mice; (L and N) IL-1 family genes in islets and liver; (O) cell-cycle genes in islets; (P) islet genes. (M) Plasma IL-1Ra in 52-week-old IL-1beta ko and control WT mice. All data were expressed per mouse.

Statistics: (A, F-H) two-way ANOVA and Sidak's multiple comparison test; (B–D, and J–P) Student's t test; error bars represent SEM, ★p < 0.05, ★★p < 0.01, ★★★p < 0.001, #p < 0.05, ###p < 0.001. See also Figures S2A–S2C.