Table 3:
Dam and Pup Serum PFAS Concentrations.
| SD+Veh | HFD+Veh | SD+PFOA | HFD+PFOA | SD+PFOS | HFD +PFOS | SD+PFHxS | HFD+PFHxS | SD+PFAS mix | HFD+PFAS mix | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
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| Dams | PFOA (μg/mL) | <LLOQ | <LLOQ | 115.2± 9.23 | 139.2± 8.43 | - | - | - | - | 145.9± 18.1 | 114.7± 8.23 |
| PFOS (μg/mL) | <LLOQ | <LLOQ | - | - | 7.32± 0.82 | 9.84± 0.61* | - | - | 4.27± 0.31# | 6.87± 0.82*# | |
| PFHxS (μg/mL) | <LLOQ | <LLOQ | - | - | - | - | 212.9± 16.2 | 255.6± 25.2 | 223.7± 36.2 | 216.6± 15.9 | |
|
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| Pups at PND 21 ** | PFOA (μg/mL) | <LLOQ | <LLOQ | 11.73± 1.42 | 17.51± 1.41* | - | - | - | - | 11.47± 0.94 | 15.21± 1.35 |
| PFOS (μg/mL) | <LLOQ | <LLOQ | - | - | 0.61± 0.15 | 0.46± 0.04 | - | - | 0.28± 0.01# | 0.39± 0.10 | |
| PFHxS (μg/mL) | <LLOQ | <LLOQ | - | - | - | - | 27.97± 2.09 | 24.10± 1.69 | 24.41± 2.78 | 25.67± 2.74 | |
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| Male Pups at PND 90 | PFOA (μg/mL) | <LLOQ | <LLOQ | 1.08± 0.26 | 1.89± 0.37* | - | - | - | - | 0.37± 0.03 | 1.80± 0.06* |
| PFOS (μg/mL) | <LLOQ | <LLOQ | - | - | 0.12± 0.03 | 0.11± 0.01 | - | - | 0.10± 0.01 | 0.11± 0.01 | |
| PFHxS (μg/mL) | <LLOQ | <LLOQ | - | - | - | - | 3.51± 0.16 | 3.82± 0.97 | 2.32± 0.42 | 3.00± 0.28 | |
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| Female Pups at PND 90 | PFOA (μg/mL) | <LLOQ | <LLOQ | 0.16± 0.04¶ | 2.00± 0.40* | - | - | - | - | 0.18± 0.08 | 1.35± 0.27* |
| PFOS (μg/mL) | <LLOQ | <LLOQ | - | - | 0.11± 0.01 | 0.20± 0.01*¶ | - | - | 0.09± 0.01 | 0.15± 0.02* | |
| PFHxS (μg/mL) | <LLOQ | <LLOQ | - | - | - | - | 3.02± 0.26 | 4.75± 0.46* | 2.22± 0.45 | 4.09± 0.38* | |
Timed-pregnant female CD-1 mice were dosed with either vehicle (0.5% Tween 20 in water), 1 mg/kg PFOA, 1 mg/kg PFOS, 1 mg/kg PFHxS, or a PFAS mix (1 mg/kg of each PFOS, PFOA, and PFHxS) via oral gavage (10 mL/kg) from gestation day (GD) 1 through postnatal day (PND) 21. PFAS were extracted from dams and pup serum collected at PND 21, and pups at PND 90. PFAS were quantified using LC-MS/MS. All mice that were not dosed with PFAS had concentrations below the lower limit of quantification (LLOQ). LLOQ were 5 ng/mL for PFOA, 1 ng/mL for PFOS, and 15 ng/mL for PFHxS. Calculations were performed using a one-way ANOVA followed by Fisher’s LSD test.
no differences were observed between male and female pups for this timepoint, and data presented represent the average of both male and female pups per litter. All values are means ± SEM; N = 3–5 litters.
p<0.05 between diet treatment within the same compound (i.e., SD+PFOS vs PFOS HFD)
p<0.05 between single compound treatment and the PFAS mixture with same diet (i.e., SD+PFOS vs SD+PFAS mix).
p<0.05 verse males of the same treatment group.