Figure 6. Endothelial cell optoeffector and optosensor expression.

(A) X-gal staining of heart cryosections from Cdh5^BAC-Optoα1AR_IRES_lacZ, Cdh5^BAC-Optoß2AR_IRES_lacZ, and Cdh5^BAC-CatCh2_IRES_lacZ mice. Scale bars: 200 µm. (B) Native fluorescence of GCaMP8 protein in heart cryosection (left panel) and brain dorsal surface (right panel). Scale bar: 200 µm. (C) Intravital two-photon imaging of Cdh5 ^BAC-GCaMP8 mice shows endothelial GCaMP8 (green) labeling of coronary microvasculature. Intravenous Texas Red-conjugated 70 kDa dextran identifies the vessel lumen (magenta). All images shown are representative images from three animals unless otherwise specified.

Figure 6—figure supplement 1. Cdh5^BAC lines.

(A) X-gal staining of brain blood vessel from Cdh5 optoeffector mice. (B) X-gal staining of vasculature in cremaster muscle and lung cryosection from Cdh5^BAC-Optoα1AR mouse. Left panel: cremaster staining; right panel: staining of lung vasculature. Scale bar: 200 µm. (C) Native fluorescence image from a non-transgenic litter mate shows no GCaMP8 fluorescence compared to transgenic litter mate in Figure 6B. Scale bar: 200 µm. All images shown are representative images from three animals unless otherwise specified.