Skip to main content
. 2021 Oct 29;10:e67858. doi: 10.7554/eLife.67858

Figure 8. Characterization of other CHROMus mouse lines.

(A) Anti-GFP immunohistochemistry (IHC) of Gja5BAC-GCaMP_GR mouse. The modified GCaMP3 protein is expressed in the atrioventricular junction (AVJ) region, atrial myocytes (AM) (left panel) and cells lining the endocardium in the adult heart (middle panel; scale bars: 200 µm) and the renal artery (right panel; scale bar: 60 µm). (B) X-gal staining of adult brain sagittal section from DbhBAC-CatCh2_IRES_lacZ mouse showing LacZ expression in the locus coeruleus region. Box outlines area of enlargement. CB: cerebellum; LC: locus coeruleus. Scale bars; top panel: 500 µm; bottom panel: 200 µm. (C) X-gal staining of cerebellar region from Aldh1l1BAC-Optoα1AR_IRES_lacZ mouse. Box outlines area of enlargement. Scale bars; top panel: 2 mm; bottom panel: 200 µm. (D) X-gal staining of spleen from LckBAC-Optoα1AR_IRES_lacZ mouse. Inset image shows X-gal staining from a wildtype littermate control. Scale bars: 300 µm; inset: 600 µm. (E) Anti-GFP IHC of lung, trachea, and testis tissues from Foxj1BAC-GCaMP8.1 mouse. Scale bars: 200 µm. All images shown are representative images from three animals unless otherwise specified.

Figure 8.

Figure 8—figure supplement 1. Transgene expression in other CHROMus lines.

Figure 8—figure supplement 1.

(A) Anti-GFP immunohistochemistry (IHC) of aorta from Gja5BAC-GCaMP_GR mouse demonstrates endothelial GCaMP5 expression. Scale bar: 200 µm. (B) X-gal staining of adrenal gland from a DbhBAC-CatCh2_IRES_lacZ mouse showing LacZ expression in the adrenal medulla. Box indicates area of enlargement. Scale bars: left panel , 500 µm; right panel, 200 µm. (C) X-gal staining of kidney (left panel) and olfactory lobe (right panels) from Aldh1l1BAC-Optoα1AR_IRES_lacZ mouse. Box indicates area of enlargement. Scale bars: upper panel, 200 µm; lower panels, 600 and 300 µm. (D) GCaMP8 expression in lung from SftpcBAC-GCaMP8 mice consistent with a type II alveolar cell pattern. Scale bar: 300 µm. All images shown are representative images from three animals unless otherwise specified.