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. 1973 Sep 1;14(3):474–488. doi: 10.1186/BF03547434

Serological Cross-Reactions Between Different Brucella Species and Yersinia Enterocolitica

Agglutinating Activity Of 19 S and 7 S Antibodies Against Somatic Antigen of Brucella Abortus and Yersinia Enterocolitica Type IX

Serologiska korsreaktioner mellan olika Brucella species och Yersinia enterocolitica. Agglutinationsaktiuitet hos 19 S och 7 S antikroppar mot somatiska antigener hos Brucella abortus och Yersinia enterocolitica typ IX

B Hurvell 1,✉
PMCID: PMC8559810  PMID: 4128117

Abstract

Antisera from rabbits immunized with acetone-killed whole cells and with lipopolysaccharides from Brucella abortus (B.a.) and Yersinia enterocolitica type IX (Y.e.) were gel-filtered on Sephadex G-200 columns.

All the antisera had serologically cross-reacting agglutinins against B.a. and Y.e. both in the 19 S and in the 7 S antibody fractions.

The homologous and the heterologous agglutinating activity of 19 S and of 7 S antibodies was tested before and after treatment with dithiothreitol (DTT) and subsequent alkylation. Unlike 19 S antibodies, 7 S antibodies were resistant to the DTT reduction.

The results from heterologous absorptions of the respective 19 S and 7 S antibody fractions indicated that 19 S antibodies both to B.a. and to Y.e. had a greater tendency towards being absorbed to the cross-reacting antigenic determinants than had the corresponding 7 S antibodies. The agglutination titres for 19 as well as 7 S antibody fractions derived from the immunizations with B.a. (both whole cells and LPS) fell more markedly after heterologous absorptions than did the analogous titres for corresponding Y.e. antibody fractions. Possible explanations of these differences are discussed.

Keywords: serological cross-reactions, Brucella abortus, Yersinia enterocolitica serotype IX, 19S antibodies, 7 S antibodies, agglutination

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